How to use statistical analysis

Overview of statistical analysis result page 2

A.	Summary of information
	Length of the target genome sequence, number of CpG sites and number of bisulfite sequences are indicated.
B.	Statistical data
	Position of CpG sites, methylation status of each CpG sites and statistical significances (P-value) of difference between two bisulfite sequence groups are shown. Fisher's exact test The statistical significance of the difference between two bisulfite sequence groups at each CpG site is evaluated with Fisher's exact test that is non-parametric statistical significance test to determine if there are nonrandom associations between two categorical data. See more detail. Mann-Whitney U-test The statistical significance between two groups of the entire set of CpG sites is evaluated with the Mann-Whitney U-test (also called the Wilcoxon rank-sum test) that is non-parametric statistical significance test for two distributed samples. See more detail. As a limitation of both tests, CpG methylation pattern is not considered and allele specific CpG methylation pattern, especially for imprinting locus, is not detectable. Figure of comparative methylation status is also shown.