Conditions to exclude bisulfite sequences
If you want, change conditions to exclude low quality bisulfite sequences.
⋅Upper limit of unconverted CpHs (
number of unconverted CpHs (CpA, CpC, CpT)
)
⋅Lower limit of percent converted CpHs (
percent of "number of converted CpHs"/"number of CpHs"
)
⋅Upper limit of alignment mismatches (
number of alignment mismatches and gaps between genomic and bisulfite sequences
)
⋅Lower limit of percent identity (
percent of alignment identity between genomic and bisulfite sequences
)
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