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QUMA: QUantification tool for Methylation Analysis
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You can easily align, visualize and quantify
bisulfite sequence data for CpG methylation analysis

 list  Overview: how to use QUMA space  list  Quick start space  list  Execute with sample sequence data space  list  Sample sequence files
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 list  Upload target genomic sequence (PCR target) file
(plain sequence, FASTA or GenBank format)
The genomic sequence must be an unconverted sequence between PCR primer pair (not necessary to convert "C" to "T").
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 list  Upload bisulfite sequence file
(multi-FASTA format file or zipped archive of sequence files)
Raw sequence data can be used. Removal of plasmid vector sequence is not necessary.
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help
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 arrow    Show options
space Go to Home PagespaceQUMA (QUantification tool for Methylation Analysis) top
 list  Upload QUMA restore file (.qma) to restore the analysis space   help
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    What's New
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  • Jul 19 2014, Jan 16 2014, Apr 9 2013
    • Minor modification
  • Oct 25 2012
    • Minor bug fixed
  • Aug 23 2012
    • ABI format file (with ".ab1" extension) is now acceptable for bisuflite sequece file
  • May 23 2012
    • Exclude identical bisulfite sequences option is added (Click above 'Show options' to display)
  • Previous News
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    Supported browsers
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We support the following web browsers;
  • Firefox (Mac/Win)
  • Safari (Mac)
  • Opera (Mac/Win)
  • Internet Explorer(IE) 6.0 and higher (Win)  (IE 7/8 is not recommended because it has many bugs)
Many browsers such as IE 5.0 for Windows, Mozilla, and Netscape 6 and higher may work as well.
Some older browsers such as IE for Mac or Netscape 4 will not work.
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    Contact information
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  Yuichi Kumaki* & Masaki Okano
  Center for Developmental Biology, RIKEN
  2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
  * Present address for Yuichi Kumaki:
  Department of anatomy and cell biology, Graduate school of medicine,
  Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
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spacer Center for Developmental Biology, RIKEN
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If you have any questions/comments/requests etc., please feel free to contact  : 
quma@cdb.riken.jp
If you link to QUMA web site, please use this URL http://quma.cdb.riken.jp/
Copyright© 2008-2014 RIKEN Center for Developmental Biology All rights reserved.
Last updated at Sat Jul 19 00:30:00 JST 2014 spacer QUMA (QUantification tool for Methylation Analysis) top