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| Reason for the exclusion |
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| When bisulfite sequence is excluded, reason(s) for the exclusion will be indicated. Conditions to exclude low quality bisulfite sequences can be changed (Click "Show options" link to show the optional parameters). |
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mismatch:
the number of alignment mismatches (includes gaps) between genomic and bisulfite sequences exceeded the upper limit (default: 10). This means low quality sequence read. |
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% ident:
percent of alignment identity between genomic and bisulfite sequences exceeded the lower limit (default: 90%). This means low quality sequence read. |
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unconv:
the number of unconverted CpHs (CpA, CpC and CpT) exceeded the upper limit (default: 5). This means incomplete bisulfite conversion or low quality sequence read. |
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% conv: percent of "number of converted CpHs" / "number of CpHs" exceeded the lower limit (default 95%). This means incomplete bisulfite conversion or low quality sequence read. |
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multiple: identical bisulfite sequence at the target region exists. |
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user desired: sequence was excluded by checking on the "exclude" checkbox. |